Tlhatlhobo ea Lactoperoxidase

Lactoperoxidase (LPO), e fumanoang litšoeleng tsa lefutso le mammary, ke karolo ea bohlokoa ea karabelo ea 'mele ea ho itšireletsa mafung ho boloka bophelo bo botle ba molomo. Karolo ea bohlokoahali ea lactoperoxidase ke oxidize thiocyanate ions (SCN−) e fumanoang mathe boteng ba hydrogen peroxide e hlahisang lihlahisoa tse bonts'ang mesebetsi ea antimicrobial. LPO e fumanoeng lebeseng la bovine e sebelisitsoe indastering ea bongaka, ea lijo le ea litlolo ka lebaka la ts'ebetso ea eona e tšoanang le sebopeho sa sebopeho sa motho.

Lihlahisoa tsa sejoale-joale tsa bohloeki ba molomo li ntse li ruisoa le sisteme ea lactoperoxidase ho fana ka mokhoa o mong o motle ho menoana e tloaelehileng ea fluoride. Ka lebaka la lits'ebetso tse pharaletseng tsa tlatsetso ea lactoperoxidase, tlhoko ea eona e eketsehile haholo ho theosa le lilemo, 'me e ntse e hola.
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Lactoperoxidase ke eng?

Lactoperoxidase ke enzyme feela ea peroxidase e hlahisoang ho tsoa ho mucosal, mammary le galavary gland, e sebetsang joalo ka moemeli oa tlhaho oa anti-bacterial. Ho batho, encyme ea lactoperoxidase e kentsoe ke mofuta oa LPO. Enzyme ena hangata e fumanoa ho liphoofolo tse anyesang, ho kenyelletsa batho, litoeba, bo-bovine, kamele, buffalo, khomo, poli, Ilama le linku.

Mosebetsi oa Lactoperoxidase:

LPO ke moemeli ea sebetsang hantle oa lithibela-mafu. Lisebelisoa tsa Lactoperoxidase, ka hona, li thehiloe molemong ona. Ka hona, ts'ebeliso ea lactoperoxidase e fumaneha haholo poloko ea lijo, tharollo ea mahlo le merero ea litlolo. Hape, lactoperoxidase phofo e sebelisitsoe kalafo ea maqeba le ea meno. Ntle le moo, LPO ke moemeli ea sebetsang oa li-anti-viral le anti-tumor. Ho tšohloa ka tlase mona ho sebelisoa lactoperoxidase haholoanyane:

i. Kankere ea matsoele

Kankere ea Lactoperoxidase bokhoni ba taolo bo amahanngoa le matla a eona a oxidize estradiol. Oxidation ena e lebisa khatellong ea oxidative liseleng tsa mofetše oa matsoele. Ts'ebetso ea Lactoperoxidase mona ke ho baka methapo e mengata e lebisang ho tšebeliso ea oksijene le ho bokellana ha hydrogen perrogen. Ka lebaka la liphetoho tsena, LPO e bolaea lisele tsa tumor ka tsela e hlakileng. Hape, macrophages a pepesitsoeng LPO a etsoa ts'ebetso ea ho senya lisele tsa mofetše, a li bolaea.

Ii. Matla a khahlanong le vaerase

Enzyme ea LPO e sebetsa e le sebopeho sa tlhaho sa ts'ireletso ea likokoana-hloko tse sa sirelng 'mele ea tlhaho' me e tsibatsa oxidation ea thiocyanate ion ho antihacterial hypothiocyanate. LPO e ka thibela kholo ea likokoana-hloko tse ngata ka karabelo ea enzymatic e kenyang li-ion tsa thiocyanate le hydrogen peroxide e le li-cofactors. Ts'ebetso ea antimicrobial ea LPO e bohlokoa ho theho ea li-ion tsa hypothiocyanite ka ts'ebetso ea li-enzyme. Li-ion tsa Hypothiocyanite li khona ho sebetsana le litho tsa kokoana-hloko. E boetse e baka tšitiso mosebetsing oa li-enzyme tse itseng tsa metabolic. Lactoperoxidase e bolaea libaktheria tse se nang Gram mme e thibela kholo le nts'etsopele ea libaktheria tse ntle tsa Gram.

Iii. Lactoperoxidase ho litlolo

Motsoako oa phofo ea lactoperoxidase, tsoekere, thiocyanate, iodide,

le oxidase ea tsoekere, 'me li tsejoa li sebetsa hantle ho bolokoseng litlolo.Lactoperoxidas-02

Iv. Lactoperoxidase ka lebese Ho sireletsoa

Bokhoni ba lactoperoxidase ts'ebetsong ea ho boloka boleng bo hloekileng ba lebese le tala ka nako e itseng e thehiloe libakeng tse 'maloa le lithuto tsa liteko tse entsoeng libakeng tse fapaneng tsa sebaka. Lactoperoxidase e bolokiloeng e ka sebelisoa ho boloka lebese le tala le fumanoang mefuteng e fapaneng. Hore na mokhoa o sebetsa hantle hakae ho latela lintlha tse ngata. Lintho tsena li kenyelletsa mocheso oa lebese nakong ea kalafo, mofuta oa tšoaetso ea kokoana-hloko le bongata ba lebese.

Lactoperoxidase e fana ka phello ea bacteriostatic lebeseng le tala la liphoofolo tse anyesang. Lintlha tsa boithuto le boiphihlelo bo tsoang lits'ebetsong tsa 'nete li bonts'a hore lactoperoxidase e ka sebelisoa ho feta moeli oa mocheso oa (15-30 degrees Celsius) o boletsoeng ho li-codex tsa tataiso tsa 1991. Qetellong ea tekanyo ea mocheso, lithuto tse fapaneng li bonts'a ts'ebetso ea lactoperoxidase e ka liehisa kholo ea baktheria ea lebese ea psychrotrophic mme ka hona ea lieha ho senya lebese matsatsi a mangata ha a bapisoa le sehatsetsi feela. Ho bohlokoa ho hlokomela hore sepheo sa ho sebelisa lactoperoxidase ha se ho etsa hore lebese le sireletsehe bakeng sa tšebeliso empa ho boloka boleng ba lona ba mantlha.

Ho etsa bohloeki bo botle ho hlahiseng lebese ho bohlokoa ts'ebetsong ea lactoperoxidase le boleng ba lebese la microbiological. Tšireletseho le boteng ba lebese li ka fumaneha feela ka ho kopanya mocheso oa lebese le mekhoa e metle ea bohloeki e ikemetseng ntle le ho sebelisa lactoperoxidase.

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v. Mesebetsi e meng

Boithuto bo bonts'itse hore ntle le ho ba le litlamorao tsa ts'oaetso, Lactoperoxidase e ka sireletsa lisele tsa liphoofolo liphellong tse fapaneng le peroxidation, hape ke karolo ea bohlokoa ea ts'ireletso khahlanong le likokoana-hloko tsa pathogenic tsamaisong ea tšilo ea masea a sa tsoa hlaha.

Sisteme ea Lactoperoxidase

Sisteme ea Lactoperoxidase ke eng?

Lactoperoxidase system (LPS) e entsoe ka likarolo tse tharo, tse kenyeletsang lactoperoxidase, hydrogen peroxide, le thiocyanate (SCN¯). Sisteme ea Lactoperoxidase e na le ts'ebetso e khahlanong le baktheria ha feela likarolo tsena tse tharo li sebetsa 'moho. Ts'ebelisong ea nnete ea bophelo, haeba ts'oaetso ea karolo e itseng tsamaisong e sa lekana, e tlameha ho eketsoa ho etsa bonnete ba hore anti-bacteric athari, e tsejoang ka hore ke ts'ebetso ea LPS. Har'a bona, mahloriso a lactoperoxidase ha ea lokela ho ba tlase ho 0.02 U / mL.

Khetla ea tlhaho ea lactoperoxidase ka lebeseng la bovine ke 1.4 U / mL, e ka fihlelang tlhokahalo ena. SCN¯ e fumaneha ka bongata mekheng le liphoofolong tsa liphoofolo. Ka lebese, khatello ea thiocyanate e nyane joalo ka μg /g / mL. Hona ke ntho e lekanyetsang ts'ebetso ea sistimi ea Lactoperoxidase. Ho 'nile ha fanoa ka tlhahiso ea hore thiocyanate e hlokahalang ho qala ts'ebetso ea lactoperoxidase e haufi le 3 μg / mL kapa le ho feta. Ke ka lebaka leo re hlokang ho kenyelletsa thiocyanate ena ea kantle ho kenya ts'ebetsong sisteme ea lactoperoxidase. Likahare tsa hydrogen peroxide ka lebese, le kentsoeng, ke 5-15 μg / Ml feela, mme ts'ebetso ea LPS e hloka 1-2 μg / mL ea hydrogen peroxide. Ke ka lebaka leo hydrogen peroxide e lokelang ho fanoa kantle ho naha.

Sisteme ea lactoperoxidase e bapala karolo ea bohlokoa tsamaisong ea 'mele ea ho itšireletsa mafung, e ka bolaea libaktheria ka lebese le mucosal le ho ba le ts'ebeliso ea kalafo.

Lihlahisoa tsa tlhokomelo ea lijo le bophelo bo botle, ho eketsoa kapa ho eketsoa hoa sisteme ea lactoperoxidase ka linako tse ling ho sebelisoa ho laola libaktheria.

Hona e sebetsa?

LPS e na le tlhahiso ea motsoako o khahlanong le baktheria o tsoang ho SCN¯catalysed ke LPO boteng ba hydrogen peroxide. The lactoperoxidase e boletsoeng ts'ebetso ea antimicrobial e fumaneha ka tlhaho metsing a 'maloa a kang lero la lets'ollo, meokho le sesepa. Mefuta e 'meli ea bohlokoa ea sistimi ea antimicrobial, e leng hydrogen peroxide le thiocyanate, e teng ka lebese maemong a fapaneng, ho latela mofuta oa phoofolo le fepa e fanweng.

Ka lebese le lecha, tšebetso ea antimicrobial ha e na matla ebile e lula e le lihora tse peli kaha lebese le na le "hydrogen peroxide" le "thiocyanate" feela. Thiocyanate ea eketsoa e kopantsoeng le "2 electron reaction" e hlahisang hypothiocyanite

Thiocyanate e sebetsa e le cofactor bakeng sa Sisteme ea Lactoperoxidase. Ka lebaka leo, palo ea li-sulfhydryls tse nang le oksijene ka kakaretso e ikemetse ea thiocyanate ion halelele

  1. Boloetse ba boithabiso bo teng
  2. Thiocyanate ha e khathale
  • Ho na le hydrogen peroxide e lekaneng
  1. Thiocyanate ha e e-so ka e kenngoa ho amino acid e nkhang hamonate

Ka lebaka leo, thiocyanate e nchafatsa phello ea anti-bacterial ea sisteme ea lactoperoxidase ka lebese le lecha. Sena se holisa bophelo ba mabenkele a lebese le lecha tlasa maemo a chesang a mongobo ka lihora tse supileng ho isa ho tse robeli.

Sesebelisoa sa Lactoperoxidase / Sebelisa

i. Ketso e khahlanong le likokoana-hloko

Ts'ebetso ea anti-microbial ea sisteme ea lactoperoxidase e bonoa ketsong ea bactericidal le bacteriostatic ea likokoana-hloko tse ling tse fumanehang lebeseng le tala. Mochine oa eona oa bactericidal o sebetsa ho hore sehlopha sa thiol se fumanehang molekong oa plasma ea lisele tse nyenyane se hlahisoa ka bongata. Sena se baka tšenyo ea sebopeho sa membrane ea plasma e lebisang ho lutleng ea polypeptides, ion ea potasiamo le li-amino acid. Ho bokelloa ha morara le li-pyrimidine, tsoekere le amino acid ka lisele ho thibetsoe. Synthesis ea DNA, RNA, le liprotheine le tsona ha lia thibeloa.

Libaktheria tse fapaneng li bontša likhahla tse fapaneng tsa kutlo ea sisteme ea lactoperoxidase. Libaktheria tse senang moea oa gram, tse kang Salmonella, Pseudomonas le Escherichia coli, li thibelloa ebile lia bolaoa. Libaktheria tsa lactic acid le Streptococcus ha lia thibeloa feela. Ho senngoa ha libaktheria tsena ke sisteme ea lactoperoxidase ho baka ho lutla hoa limatlafatsi tse ling, ho sitisa libaktheria ho ja limatlafatsi, 'me sena se lebisa ho theoheng kapa ho bolaeng ha libaktheria.

Ii. Phekolo ea paradentosis, gingivitis, le ho bolaea lisele tsa tumor

LPS ho lumeloa hore e sebetsa hantle kalafong ea gingivitis le paradentosis. LPO e sebelisitsoe ka har'a rinse ea molomo ho theola libaktheria tsa molomo, ka lebaka leo, asiti e hlahisoang ke libaktheria tsena. Li-anti-conjugates tsa sisteme ea lactoperoxidase le gluidase oxidase li bontšitse hore li sebetsa hantle ho senya mme ka hona li bolaea lisele tsa tumor ho vitro. Hape, macrophages a pepesetsoeng sisteme ea lactoperoxidase e etsoa ts'ebetsong ho senya le ho bolaea lisele tsa mofetše.

Iii. Tlhokomelo ea Mantsoe

Boithuto bo fapaneng ba kliniki bo hlalosang katleho ea LPS ho menoana ea meno bo ngotsoe. Kamora ho bonts'a ka mokhoa o sa tobang, ho sebelisa maemo a liteko tsa caries ea litekanyetso, hore menoana ea lactoperoxidase e nang le amyloglucosidase (γ-amylase) e na le litlamorao tse ntle tsa tlhokomelo ea molomo. Li-Enzymes tse kang glucose oxidase, lysozyme, le lactoperoxidase li fetisoa ka kotloloho ho tloha leino la meno ho ea ho pellicle.

Kaha li entsoe ka pellicle, li-enzyme tsena li sebetsa ka mokhoa o matla haholo. Hape, LPS e na le tšusumetso e ntle ea ho thibela khahlanong le li-caries tsa bongoana tsa bongoana ka ho fokotsa palo ea likolone tse thehiloeng ke microflora ea cariogenic ha e ntse e eketsa khatello ea thiocyanate.

Le bakuli ba xerostomia, lactoperoxidase leino le opang e phahame haholo ha e bapisoa le leino la meno la fluoride ha ho tluoa mokhoeng oa ho rala. Ts'ebeliso ea LPS ha e felle feela ho periodontitis le caries. Motsoako oa "lactoperoxidase" le "lysozyme" o ka sebelisoa kalafong ea kalafo ea molomo.

Ha LPS e kopantsoe le lactoferrin, motsoako ona o loantša halitosis. Ha LPS e kopantsoe le lysozyme le lactoferrin, LPS e thusa ho ntlafatsa matšoao a xerostomia. Hape, li-gels tse nang le lactoperoxidase system li thusa ho ntlafatsa matšoao a kankere ea molomo ha tlhahiso ea mathe e thibeloa ka lebaka la ho thulana.

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Iv. Ho ntlafatsa sesole sa 'mele

Ketsahalo ea antimicrobial ea lactoperoxidase e bapala karolo ea bohlokoa sesole sa 'mele. Hypothiocyanite ke karolo e sebetsang e hlahisoang ke mosebetsi oa lactoperoxidase ho thiocyanate. Hydrogen peroxide e hlahisoa ke liprotheine tsa Duox2 (oxidase 2). Sesocyanate secretion ho bakuli ba nang le cystic fibrosis e theoleloa. Sena se fella ka phokotso tlhahiso ea antimicrobial hypothiocyanite. Sena se beha kotsing e kholo ea tšoaetso ea moea.

LPS e thibela helicobacter pylori hantle. Empa ka bongata bohle ba batho, LPS e bonts'a matla a fokolang a anti-bacterial. LPS ha e hlasele DNA ebile ha e mutagenic. Empa, tlasa maemo a itseng, LPS e ka baka khatello e tlase ea oxidative. Ho pakoa hore LPO boteng ba thiocyanate e ka baka litlamorao tsa cytotoxic le bactericidal tsa hydrogen peroxide tlasa maemo a itseng, ho kenyeletsoa ha H2O2 e le teng ka har'a motsoako oa karabelo e fetang thiocyanate.

Ntle le moo, ka lebaka la thepa ea eona e matla le e sebetsang ea antibacterial le ho hanyetsa mocheso o phahameng, e sebelisoa e le moemeli oa antibacterial bakeng sa ho fokotsa libaka tsa baktheria lihlahisoa tsa lebese kapa tsa lebese le joalo ka sesupo sa lebese la ultra-pasteurization. Ka ho kenya tšebetsong sisteme ea lactoperoxidase, bophelo ba sehatsetsing lebese le tala le phehiloeng le bona bo ka eketsoa.

Hape, hypothiocyanate e hlahisoang ke lactoperoxidase e ka sebelisoa ho thibela vaerase ea herpes rahisix le vaerase ea immunodefitness ea motho.

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Na ho bolokehile bakeng sa bophelo ba batho le ba liphoofolo?

Lilemo tse leshome le metso e mehlano tsa lithuto tsa masimong linaheng tse tsoelang pele le tse tsoetseng pele li ile tsa etsoa tsa ba tsa hlahlojoa ke FAO / WHO JECFA (Komiti ea Litsebi tsa Joint ka Lisebelisoa tsa Lijo). Kamora hore lithuto tsena tse tebileng le tse kholo li phetheloe, ts'ebeliso ea sisteme ea lactoperoxidase ho boloka lebese e amohetsoe ke FAO / WHO JECFA (Komiti ea Litsebi mabapi le Lisebelisoa tsa Lijo). Litsebi li boetse li phatlalalitse hore mokhoa ona o sireletsehile bakeng sa bophelo ba batho le ba liphoofolo.

LPS ke sebaka sa tlhaho sa lero la gastric le mathe ho batho, ka hona, se sireletsehile ha se sebelisoa ho latela Litataiso tsa Codex Alimentarius Commission. Mokhoa ona ha o ame liphoofolo tse anyesang leha e le life. Lebaka ke hobane kalafo e etsoa feela kamora hore lebese le ntšoe letlalong.

fihlela qeto e

Ho bonahala ho tsoa puisanong ea rona hore sisteme ea lactoperoxidase le lactoperoxidase e sebetsa haholo ebile e na le thuso ea mefuta e mengata ea likopo. Haeba u batla ho etsa se phethahetseng lactoperoxidase reka bakeng sa lipatlisiso tsa hau kapa nts'etsopele ea lithethefatsi, se ke oa sheba hole. Re na le bokhoni ba ho etsa li-lactoperoxidase tse ngata ka nako e khuts'oane ka ho fetisisa ebe re li romella US, Europe, Canada le libakeng tse ling tsa lefats'e. Ikutloe u lokolohile ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi.

References

  1. Jantschko, PG Furtmüller, M. Allegra et al. "Redox intermediates of limela le mammonal peroxidases: thuto e ts'oanang ea thuto ea nakoana ea ts'ebetso ea bona e sebetsang hape mabapi le lits'oants'o tsa indole," Archives of Biochemistry and Biophysics, vol. 398, che. 1, maq. 12-22, 2002.
  2. Tenovuo JO (1985). "Sistimi ea peroxidase mecheng ea batho." Ho Tenovuo JO, Pruitt KM (eds.). Sisteme ea Lactoperoxidase: chemistry le bohlokoa ba likokoana-hloko. New York: Dekker. leq. 272.
  3. Thomas EL, Bozman PM, Ithute DB: Lactoperoxidase: sebopeho le thepa e thusang. Peroxidases ho Chemistry le Biology. E hlophisitsoe ke: Everse J, Everse KE, Grisham MB. 1991, Boca Raton, FL. CRC Press, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner GE (Phato 2003). "Lactoperoxidase le ts'ireletso ea moea oa batho e tsamaisang batho". Am. J. Respir. Cell Mol. Biol. 29 (2): 206–12.
  5. Mikola H, Waris M, Tenovuo J: Thibelo ea mofuta oa vaerase ea herpes rahisix, vaerase ea phefumoloho, le mofuta oa echovirus 1 ke hypothiocyanite e hlahisoang ke peroxidase. Pholoso ea Ts'oaetso. 11, 1995 (26): 2-161.
  6. Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (Lwetse 2004). "Boikutlo ba Helicobacter pylori ho ts'ireletso ea kahare ea ts'ireletso, sisteme ea lactoperoxidase, e buffer le matheong a batho kaofela". Tlaleho ea bongaka ba Microbiology. 53 (Pt 9): 855-60.

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